Streak plate method is a pure culture technique which helps in isolating desired colonies. Sep 05, 2014 a fundamental skill that a student must have is the ability to isolate pure cultures. The most common method of separating bacteria cell on agar surface to obtain isolated colonies is the streak method to inoculate petri dish. The streak plate method is a rapid qualitative isolation method for obtaining discrete colonies from a mixed population. Streak plate methods of isolation medical laboratories. The inoculum is streaked over the agar surface to isolate colonies on at least a portion of the plate. Eventually, it will wear out and you need to change it every once in a while. Vdyoutube blog how to download video and audio files from. It is essentially a dilution technique that involves spreading a loopful of. Serial dilution is the simplest technique for obtaining manageable concentrations of a desired organism and it is.
Principle the streak plate technique is essentially a method to dilute the number of organisms, decreasing the density. Here you will find lots of video links to help you practice some technique moves at home. Jan 03, 2007 a streak plate is used to identify a bacterial species, you can identify a particular species by altering the nutrients in the agar which you use. His recording career began with two singles released on prep records, followed by a short stint with capitol records.
The techniques commonly used for isolation of discrete colonies initially require that the number of organisms in the inoculums be reduced. During streak ing, the cell density decreases, eventually leading to individual cells being deposited separately on the agar surface. Sometimes a solution of bacteria is too concentrated to work with. In streaking microbiology, the plate used to incubate a culture and isolate a pure strain. A commonly used isolation technique is the streak plate. Sign in to view the content amrita vishwa vidyapeetham. This technique involves streaking four sides of a plate, sterlizing the loop between each quadrant and reinoculating it by touching an edge of each quadrant and dragging it to streak the next streak plate technique. But there is no clear answer about how it is done like how to find the link of the video file and download it. This allows for individual colonies to be isolated from other colonies. Facebook linkedin twitter youtube contact jove recommend jove. Facebook linkedin twitter youtube contact jove recommend jo ve. In the streak plate method of isolation, a bacterial sample always assumed to be a mixed culture is streaked over the surface of a plated agar medium.
Learn streak plate method with free interactive flashcards. Apr 22, 2015 isolation streak plate technique video tutorial from worcester polytechnic. His recording career began with two singles released on prep records, followed by a short stint with capitol. May 19, 2008 how to use the streak plate technique to cultivate bacterial cultures. Cleaning glass is more about the tools than the elbow grease. Feb, 2010 for a streak plate you streak the sample across one edge of the plate using the swab if that is how the sample came, or a sterile loop if the sample is a liquid or spreadable slurry or paste. Some individual bacterial cells are separated and well spaced from each other. There are 81 videos about streaking on vimeo, the home for high quality videos and the people who love them. Streak plating is an extremely effective way of generating. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed. The streak plate technique is based on progressive dilution of a sample figure 1, with the.
Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. A common method for the isolation of a pure culture from a mixture is by streaking plates. Vdyoutube blog how to download video and audio files. Streak plate virtual interactive bacteriology laboratory. Want to save youtube video to your mac for play offline. The task takes on a new level of annoyance when all that careful spraying and wiping results in a. For a streak plate you streak the sample across one edge of the plate using the swab if that is how the sample came, or a sterile loop if the sample is a liquid or spreadable slurry or paste. Although not as cringeworthy as other household chores, like scrubbing down the old toilet bowl, window cleaning is nonetheless tedious and repetitive. Streaking is a technique used to isolate a pure strain from a single microorganism. Isolation of bacteria by dilution techniques flashcards. Ray stevens born harold ray ragsdale, 24th january 1939 is an american country and pop singersongwriter, best known for a series of irreverent, quirky singles such as mississippi squirrel revival and the streak.
Streak plate method of isolation purpose the streak plate technique is the most widely used method of obtaining isolated colonies from a mix of cultures. In streak mineralogy, the plate used to produce the powder of a mineral. Jul 09, 2011 for youtube srt to work, the video must have a closed caption file assigned to it in order to convert the subtitle format to srt. An old kitchen tile or even a sidewalk can also serve as a streak plate. Streak plates have a mohs hardness of around 7, but be sure to check your streak plate against a piece of quartz hardness 7 because some are softer and some harder. For youtube srt to work, the video must have a closed caption file assigned to it in order to convert the subtitle format to srt.
The streak plate method can be timeconsuming, especially if you are going to prepare a large sample size. The inoculum is streaked over the agar surface in such a way that it thins out the bacteria. Jun 17, 2016 this was what i could gather in 15 minutes. Microbial life is available from oxford university press. The streak plate method is a rapid qualitative isolation method. Then take one streak from that, another from that and another from that, sterilising the loop in a flame betwen each streak, giving four different. If you like our site and find our work useful, please consider donating, your great contributions help us to pay for the server cost, domain renewal, and other maintenance costs of this site. Get youtube link downloader now to download youtube video by pasting youtube link url. How can i make a youtube video downloader web application from scratch.
It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate. Could some bacteria grow on the streak plate but not be seen using the pour plate. Although the project we made was based on python, the. For the actor and singer with the village people, see ray stephens. What are the drawbacks of the streak plate technique. There are many similar questions like this but there is no really a. Download youtube videos directly to your android device. Streak plate definition of streak plate by merriamwebster. This disambiguation page lists articles associated with the title streak plate. New paper model science paper model brain atlas free paper craft download on papercraftsquare. Isolation of single bacterial colonies from a mixed sample.
Streak plate definition is a piece of white unglazed porcelain on which to test the streak of a mineral. The streak plate method will require constant use of the streaking loop. Isolation streak plate technique video tutorial from. Youtube video site video clips youtube downloader free. An introduction to microbiology, aseptic technique and safety. Copy and paste a youtube video url and you will be displayed with subtitles in one or more languages as available. Streak plate technique is used for the isolation into pure culture of the organisms mostly bacteria, from mixed population. There are alternative methods for preparing a streak plate but the method shown is. Plate streaking for bacterial enumeration and strain isolation. How to download subtitles from youtube videos in srt format. Technique videos leesville road middle school dance.
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